最新澳门网址大全

  • 地址:济南市槐荫区鑫苑世家公馆2号楼2603室
  • 电话:0531-87568508 /
  • 手机:18653128690
  • 邮箱:jnyhrs@163.com
  • 无菌室装修的建造条件有哪些?
    来源:最新澳门网址大全 浏览: 发布日期:2019-05-17
      1.无菌室应坚持清洁,严禁堆放杂物,以防污染。
      1. The sterile room should be kept clean and no sundries should be piled up to prevent pollution.
      2.严防一切灭菌器材和培育基污染,已污染者应中止运用。
      2. Strictly prevent contamination of all sterilizing equipment and culture media. Those who have been contaminated should stop using them.
      3.无菌室应备有工作浓度的消毒液,如5%的甲酚溶液,70%的酒精,0.1%的新洁尔灭溶液,等等。
      3. The sterile room should be equipped with disinfectant of working concentration, such as 5% cresol solution, 70% alcohol, 0.1% Bromogeramine solution, etc.
      4.无菌室应定期用适合的消毒液灭菌清洁,以保证无菌室的干净度契合请求。
      4. The sterile room should be sterilized and cleaned regularly with suitable disinfectant to ensure that the cleanliness of the sterile room meets the requirements.
      5 需求带入无菌室运用的仪器,器械,平皿等一切物品,均应包扎紧密,并应经过适合的办法灭菌。
      5. Instruments, instruments and dishes that need to be brought into sterile rooms should be tightly bandaged and sterilized by appropriate methods.
      6.无菌室运用前必需翻开无菌室的紫外灯辐照灭菌30分钟以上,并且同时翻开超净台停止吹风。操作终了,应及时清算无菌室,再用紫外灯辐照灭菌20分钟。
      6. Before using the sterile room, the ultraviolet lamp of the sterile room must be turned over to sterilize for more than 30 minutes, and at the same time, the ultra-clean table must be turned over to stop blowing. At the end of operation, the sterile room should be cleared in time and sterilized by ultraviolet lamp for 20 minutes.
      7.供试品在检查前,应坚持外包装完好,不得开启,以防污染。检查前,用70%的酒精棉球消毒表面面。
      7. Before inspecting the test products, they should insist that the outer packing is in good condition and should not be opened to prevent contamination. Before inspection, disinfect the surface with 70% alcohol cotton ball.
      8.每次操作过程中,均应做阴性对照,以检查无菌操作的牢靠性。
      8. Negative control should be done during each operation to check the reliability of aseptic operation.
      9.汲取菌液时,必需用吸耳球汲取,切勿直接用口接触吸管。
      9. When extracting bacterial liquid, it is necessary to use ear-sucking balls to absorb it. Do not directly touch the straw with your mouth.
      10.接种针每次运用前后,必需经过火焰灼烧灭菌,待冷却后,方可接种培育物。
      10. Before and after each application of the inoculant needle, it must be burned and sterilized by flame. After cooling, the inoculant can be inoculated.
      11.带有菌液的吸管,试管,培育皿等器皿应浸泡在盛有5%来苏尔溶液的消毒桶内消毒,24小时后取出冲洗。
      11. Containers with bacterium solution such as pipettes, test tubes and culture dishes should be immersed in a sterilizing barrel containing 5% Lysol solution for disinfection. They should be taken out and rinsed 24 hours later.
    无菌室装修
      12.如有菌液洒在桌上或地上,应立刻用5%石碳酸溶液或3%的来苏尔倾覆在被污染处至少30分钟,再做处置。工作衣帽等遭到菌液污染时,应立刻脱去,高压蒸汽灭菌后洗濯。
      12. If bacterial solution is sprinkled on the table or on the ground, it should be capsized at least 30 minutes with 5% carbonate solution or 3% Lysol immediately before disposal. When working clothes and hats are contaminated by bacterial solution, they should be removed immediately and washed after sterilization by high pressure steam.
      13.凡带有活菌的物品,必需经消毒后,才干在水龙头下冲洗,严禁污染下水道。
      13. Articles with living bacteria must be disinfected before they can be washed under the faucet. Pollution of the sewer is strictly prohibited.
      14.无菌室应每月检查菌落数。在超净工作台开启的状态下,取内径90mm的无菌培育皿若干,无菌操作分别注入消融并冷却至约45℃的营养琼脂培育基约15ml,放至凝固后,倒置于30~35℃培育箱培育48小时,证明无菌后,取平板3~5个,分别放置工作位置的左中右等处,开盖暴露30分钟后,倒置于30~35℃培育箱培育48小时,取出检查。100级干净区平板杂菌数均匀不得超越1个菌落,10000级干净室均匀不得超越3个菌落。如超越限度,应对无菌室停止彻底消毒,直至反复检查符合请求为止。
      14. The number of colonies should be checked monthly in the sterile room. In the open state of super-clean workbench, several sterile culture dishes with inner diameter of 90 mm were taken. The sterile operation was to inject nutrient agar culture medium of about 15 ml, which was melted and cooled to about 45 degrees C. After coagulation, the dishes were placed in incubator at 30-35 degrees C for 48 hours. It was proved that after sterilization, 3-5 plates were taken and placed in the left, middle and right parts of the working position respectively. After 30 minutes of exposure, the dishes were placed in the left, middle and right parts. The incubator was incubated at 35 C for 48 hours and removed for inspection. The number of bacteria in 100 grade clean area should not exceed one colony and 10 000 grade clean room should not exceed three colony. If exceeding the limit, sterilization of the sterile room should be stopped until repeated inspection meets the requirements.
    XML 地图 | Sitemap 地图