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  • 细胞培养实验室的无菌操作!
    来源:最新澳门网址大全 浏览: 发布日期:2019-06-14
      细胞培养实验室应能开展无菌操作、培养、准备、清洗、消毒灭菌处理、贮存等六个方面的工作。
      Cell culture laboratory should be able to carry out aseptic operation, culture, preparation, cleaning, disinfection and sterilization, storage and other six aspects of work.
      1. 无菌操作区
      1. Sterile operating area
      (1)无菌作业区:无菌作业区仅限于细胞培养等无菌作业区,好与外界隔离,不要穿越或受其他因素干扰。
      (1) Aseptic work area: Aseptic work area is limited to aseptic work area such as cell culture. It is best to isolate from the outside world and not to cross or be disturbed by other factors.
      理想的无菌手术室应分为三部分:
      The ideal sterile operating room should be divided into three parts:
      一)更衣室-换衣服,鞋子,戴帽子和面具。
      1)The dressing room - changing clothes, shoes, hats and masks.
      B) 缓冲室——位于更衣室和手术室之间。这个缓冲室的目的是确保手术室的无菌环境。同时可放置恒温箱及必要的小型仪器。
      B) Buffer room - between the dressing room and the operating room. The purpose of this buffer room is to ensure the sterile environment of the operating room. At the same time, the thermostat and necessary small instruments can be placed.
      C) 无菌手术室-专门从事无菌操作,细胞培养。尺寸适中,顶部不宜过高(不超过2.5m),以保证紫外线的有效杀菌效果;墙面光滑,无死角,便于清洁消毒。表放置不应靠墙,表应压平塑料表面,涂以白色或灰色供解剖组织使用,并观察酚红色显示pH值。
      C) Sterile operating room - specialized in aseptic operation, cell culture. Medium size, the top should not be too high (no more than 2.5m) to ensure the effective germicidal efficacy of ultraviolet radiation; smooth wall, no dead angle, easy to clean and disinfect. The tables should not be placed against the wall. The tables should be pressed flat on the plastic surface, coated with white or grey for anatomical tissue use, and observed the phenol red to show the pH value.
      (2)净化台:操作简单,安装方便,空间小,净化效果好。细菌培养室一般有两种净化台:a)侧流净化台还是立式净化台?B)外部流,或水平层流。
      (2) Purification table: simple operation, convenient installation, small space, good purification effect. There are two kinds of purification platforms in bacterial culture room: a) side flow purification platforms or vertical purification platforms? B) external flow, or horizontal laminar flow.
    无菌室装修
      2. 孵化面积
      2. Incubation area
      该区域对无菌的要求不像无菌区域那么严格,但仍然需要保持清洁和无尘,所以应该设置在干扰少的区域,而不是交通拥挤的区域。孵化可以在恒温箱或温度可控的温室中进行,这是昂贵的,通常在实验室中使用。
      The requirement for sterility in this area is not as strict as that in aseptic areas, but it still needs to be clean and dustless, so it should be set in areas with less interference, rather than in areas with heavy traffic. Incubation can be carried out in a thermostat or in a temperature-controlled greenhouse, which is expensive and is usually used in laboratories.
      3.准备区域
      3. Preparatory area
      该地区主要从事培养基及相关培养液的制备。
      This area is mainly engaged in the preparation of culture medium and related culture medium.
      4. 存储区域
      4. Storage area
      主要储存各类冰箱、干燥箱、液氮罐、无菌培养液、培养瓶等,这种环境也需要清洁无尘。
      Mainly store all kinds of refrigerators, drying boxes, liquid nitrogen tanks, sterile culture media, culture bottles, etc. This environment also needs to be clean and dust-free.
      5. 清洁消毒消毒区
      5. Clean, disinfect and disinfect areas
      清洗消毒区域应与其他区域分开,主要用于所有细胞培养容器的清洗、准备、消毒和三蒸水的准备。
      Cleaning and disinfection areas should be separated from other areas, mainly for cleaning, preparation, disinfection and preparation of three-steamed water for all cell culture containers.
    如果您有无菌室装修的需求请点击进行咨询
    If you need aseptic room decoration, please click on for consultation.

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