1. The studio should be small and flat, with an area of about 4 m2 and a height of meters. The interior decoration should be flat and smooth, without uneven or edges. The walls and roof should be made of impermeable materials to facilitate scrubbing and sterilization.
2. The indoor lighting area is large, and the indoor conditions can be seen from the outdoor.
3. In order to ensure the cleanness of the aseptic room, a buffer corridor should be set around the aseptic room, and a buffer room should be set up beside the corridor, and its area can be smaller than that of the aseptic room.
4. The sterile room, buffer corridor and buffer room are equipped with 8 light lamps and ultraviolet lamps for disinfection air. The sterilization ultraviolet lamp should be 1 m away from the working table, and its power switch should be set outdoors.
5. The entrance and exit of aseptic room and buffer room should be provided with sliding door, the door should be flush with the window, the door seam should be sealed tightly, and the two doors should be staggered to avoid air convection pollution.
6. Use and management of aseptic room
1) the sterile room should be kept clean and tidy, and only the most necessary inspection tools, such as alcohol lamp, alcohol cotton, match, tweezers, inoculation needle, inoculation ring, glass pencil, etc., should be stored in the room.
2) indoor inspection equipment and stool table shall be kept in fixed position and shall not be moved randomly.
3)每2 - 3周用2%石炭酸水溶液擦拭工作台、门、窗、桌椅及地面，然后用3%石炭酸水溶液喷雾消毒空气，紫外灯杀菌半小时。
3) wipe the workbench, doors, windows, tables and chairs and ground with 2% carbonic acid solution every 2-3 weeks, then disinfect the air with 3% carbolic acid aqueous solution, and then the ultraviolet lamp is killed for half an hour.
4 )定期检查室内空气无菌状况，细菌数应控制在10个以下，发现不符合要求时，应立即彻底消毒灭菌。无菌室无菌程度的测定方法:取普通肉汤琼脂平板、改良马丁培养基平板各3个(平板直径均9厘米)，置无菌室各工作位置 上，开盖曝露半小时，然后倒置进行培养，测细菌总数应置37°C温箱培养48小时;测霉菌数则应置27°C温箱培养5天。细菌^霉菌总数均不得超过10个为。
4) regularly check the aseptic condition of indoor air. The number of bacteria should be controlled below 10. If it does not meet the requirements, it should be disinfected and sterilized immediately. Methods: three common Broth Agar Plates and three modified Martin medium plates (9 cm in diameter) were taken from each working position of the aseptic room. The plates were exposed for half an hour after opening the cover, and then cultured upside down. The total number of bacteria should be cultured in 37 ° C incubator for 48 hours; the number of molds should be cultured in 27 ° C incubator for 5 days. The total number of bacteria and molds should not exceed 10.
5) Before sterilization in the sterile room, all items should be placed at the operation site (except for the items to be tested), and then turn on the UV lamp for sterilization for 30 minutes. When the time is up, turn off the UV lamp for standby.
6) Before entering the sterile room, the sterilized work clothes, work caps and work shoes must be changed in the buffer room.
7) the operation should be carried out in strict accordance with the aseptic operation regulations. During the operation, speak less and do not make noise, so as to keep the environment sterile.